Description: 1.4E7 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802). 1.4E7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.4E7 cells provide a method of producing pure insulin secreting cells when stimulated (please see attached protocols for the stimulation of insulin secretion). This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: Pancreatic islets

Growth Properties:

Morphology: Epitheloid

Growth Medium: RPMI-1640 + 2mM Glutamine + 10% FCS

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 26hrs. At confluence 100,000 cells/cm² can be expected.

Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

Depositor:

Originator:

References: Lieber M et al.,” (1975) Establishment of a continuous tumor cell line (panc-1) from a carcinoma of the exocrine pancreas. Int J Cancer 15: 741-747 PMID: 1140870

Hyperlink to ECACC Cell Line Data Sheet: 10070102