Description: A bromodeoxyuridine resistant mutant of 108CC5 (ECACC catalogue number 08062521)”, a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid

Also Known As:

Species: Mouse x rat hybrid

Tissue: neural

Growth Properties: Adherent

Morphology: Neuronal

Growth Medium: DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.

Release Conditions: No

Depositor: Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.

Originator: Yes

References: Heumann et al.,” (1979) Clonal hybrid cell lines expressing cholinergic and adrenergic properties. Proc. Natl. Acad. Sci. 76: 4674-4677.

Hyperlink to ECACC Cell Line Data Sheet: 08062567