1321N1
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Description
Description: 1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten (Pontén, J., Macintyre, E. H. (1968) PMID: 4313504).1321N1 has been shown to have very similar STR profile data to U-118 MG.
Also Known As:
Species: Human
Tissue: brain
Growth Properties: Adherent
Morphology: Glial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Macintyre EH, Pontén J, Vatter AE. 1972 The ultrastructure of human and murine astrocytes and of human fibroblasts in culture. Acta Pathol Microbiol Scand A. 80(2):267-83 PMID: 4337530. Macintyre EH, Wintersgill CJ, Thormar H 1972 The establishment of a line of visna virus-producing human astrocytes (V-1181N1).Med Res Eng. 11(4):7-13 PMID: 4370460.
Hyperlink to ECACC Cell Line Data Sheet: 86030402
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