Description: The cytotoxic monoclonal antibody 16.23 was obtained from a BALB/c mouse immunised with a single i.p. injection of the human melanoma cell line Mel Juso. Mouse spleen cells were removed 3 days after immunisation and fused with the myeloma P3x63Ag8.653. The Juso B cell line was typed as HLA A1,2; B7,8; CW- ; DR2,3. The monoclonal antibody 16.23 detects the conformation-dependant DR3 epitope but was also reported to react with some HLA-Dw/DRw6 individuals. Together with antibodies S2 (ECACC catalogue no. 99043002) and S5 (ECACC catalogue no. 99061827) a useful tool for the biochemical dissection of DRw6 related epitopes has been establised. It has been analysed in the 9th International Histocompatibility Workshop (9W929). The hybridoma line has been eradicated from mycoplasma using Cibrobay before depositing at ECACC.

Also Known As:

Species: Mouse

Growth Medium: RPMI 1640 + 2mM glutamine + 1mM NaP + 10% FBS

Subculturing Procedure: Maintain cultures between 3-9 x100,000 cells/ml; 5% CO2; 37°C

Release Conditions: No

Depositor: Dr. J.P. Johnson, Institute for Immunology, University of Munich

Originator: Yes

References: J. Exp Med. 1982;156:104,Immunity 1996; 4:87, J Immunol 1995; 154:2545,J Immunol 1992; 149: 754, J Immunol 148:2586, Immun Today 1989; 10:305, Hum Immunol 1986; 16:69, Eur J Immunol 1985; 15:1239, J Immunol 1985; 135:2393, J Exp Med 1985; 162:1193,”

Hyperlink to ECACC Cell Line Data Sheet: 99043001