1BR.3.G
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Description
Description: Derived from a normal fibroblast (1BR3 ECACC catalogue no. 90011801) transformed with the plasmid pSV3gpt.The genetic identity of 1BR3 (90011801)”, 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr C Arlett, MRC Cell Mutation Unit, University of Sussex
Originator: Yes
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 90020507
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