293 N3S
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.
Also Known As:
Species: Human
Tissue: Kidney
Growth Properties: Suspension
Morphology:
Growth Medium: Joklik's MEM + 2mM Glutamine + 10% Horse Serum.
Subculturing Procedure: Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO2; 37°C. Cell doubling time is 30 hours.
Release Conditions: No
Depositor: Dr F L Graham, Department of Biology, McCaster University, Ontario
Originator: Yes
References: J Gen Virol 1987; 68;937
Hyperlink to ECACC Cell Line Data Sheet: 92052131
Ask a Question
Have a question about this product? Our technical team is here to help.
