Description: The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

Also Known As: 293tsA1609neo, 293 T

Species: Human

Tissue: Kidney (foetal)

Growth Properties: Adherent

Morphology: Small slightly rounded cells growing in a dispersed manner

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 10,000 cells/cm²

Release Conditions: No

Depositor: Professor Michele Calos, Department of Genetics, Alway Building, Room M-334 Stanford University School of Medicine 300 Pasteur Drive Stanford, CA 94305-5120.

Originator: Yes

References: DuBridge RB, Tang P, Hsia HC, Leong PM, Miller JH, Calos MP. 1987 Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol Cell Biol. 7(1):379-87.PMID: 3031469.

Hyperlink to ECACC Cell Line Data Sheet: 12022001