3A sub E
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Description
Description: The cell line 3A sub E was derived from 3A(tPA-30-1)after the original line started to senesce and went through crisis. 3A subE has an unlimited lifespan and expresses human chorionic gonadotropin (hCG)
Also Known As:
Species: Human
Tissue: placenta
Growth Properties: Adherent
Morphology:
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Aspirate medium and add 0.25% trypsin/EDTA; incubate at room temperature until cells detach. Dilute with fresh medium and aliquot into new flasks at a split ratio 1:2. Renew fluid 1-2 times per week. Incubate culture at 37°C, 5% CO2 for initial 24 hours after subculture, after this at 33°C for cell growth (non-restrictive temperature). For production of hCG grow at 40°C
Release Conditions: No
Depositor:
Originator: No
References: Proc Nat Ac Sci USA 1978:75; 1409-1413
Hyperlink to ECACC Cell Line Data Sheet: 90110508
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