3T3 clone A31
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Description
Description: Cells have now lost their contact inhibition.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5×1,000 to 2×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Cell Physiol 1968;72:141; Science 1968;162:1024
Hyperlink to ECACC Cell Line Data Sheet: 86110401
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