3T3-MSV
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Description
Description: This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.
Release Conditions: No
Depositor:
Originator: No
References: Int J Cancer 1970;6:95; Proc Natl Acad Sci, USA 1978;75:4001
Hyperlink to ECACC Cell Line Data Sheet: 90092701
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