833KE
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Description
Description: A human embryonal carcinoma cell line derived from a human testicular germ cell tumour.
Also Known As:
Species: Human
Tissue: Embryonal carcinoma
Growth Properties: Adherent
Morphology:
Growth Medium: DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.
Subculturing Procedure: Split sub-confluent cultures (70-80%)”, approximately every 3-4 days, 1:3 to 1:5 i.e. seeding at more than 6 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells need to be maintained at a high cell density.
Release Conditions: No
Depositor:
Originator: No
References: Hiraoka et al.,” (1988) Ultrastructure of four human germ cell tumor-derived cell lines: effect of 12-O-tetradecanoyl phorbol-13-acetate. Cancer Invest 6:393-402.
Hyperlink to ECACC Cell Line Data Sheet: 06072611
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