8505C
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Description
Description: Established from undifferentiated thyroid carcinomas of a 78 year old female patient. Pathologically this primary carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to G:C transversion at the first base of p53 gene codon 248. Polymerase chain reaction-loss of heterozygosity assays confirmed allelic deletion of p53 gene. Loss of heterozygosity of tumour suppresser genes was not observed.
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Species: Human
Tissue: thyroid
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time is 36 hours. Saturation density at confluency is 1×100, 000 cells/cm².
Release Conditions: No
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Originator: No
References: Cancer Res 1992;52:1369; Int J Oncology 1994;4:583
Hyperlink to ECACC Cell Line Data Sheet: 94090184
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