BE11 (Early)
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Description
Description: The BE11 culture was derived 2 days after a single injection of buffer into pregnant rats. The cells do not form colonies in agar and are not tumourigenic in BDIX rats. Plasminogen activator activity is barely detectable. They are suitable controls for BE10 (Early) cells (93040118).
Also Known As:
Species: Rat
Tissue: brain
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 15-20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2×100,000 cells/cm² using 0.25% trypsin/EDTA; 10-15% CO2; 37°C. Cultures should reach 2×1,0,000 cells per 25cm² flask. Subculture weekly.
Release Conditions: No
Depositor: Dr J Roscoe, Dept. of Histopathology, University College and Middlesex School of Medicine, London
Originator: Yes
References: Br J Cancer 1980;42:756; Carcinogenesis 1986;7:477
Hyperlink to ECACC Cell Line Data Sheet: 93040121
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