C127I
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Description
Description: Non-transformed clone from a RIII mouse. Supernatant fluid is negative for RT activity. Useful for visualisation of sarcoma virus-induced foci, in vitro quantitative assay for bovine papilloma virus. Suitable host for transformation with bovine papilloma virus DNA plasmids.
Also Known As:
Species: Mouse
Tissue: mammary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:15 i.e. seeding at 1-2×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Virol 1978;26:291; Virology 1980;103:369; Mol Cell Biol 1981;1:486
Hyperlink to ECACC Cell Line Data Sheet: 90060504
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