Hep2 (Clone 2B) (HeLa derivative)
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Description
Description: This cell line has been adapted for growth in suspension. Cells support growth of arboviruses, measles virus, poliovirus 1, adenovirus 3 and VSV (Indiana strain). This cell line has been found to be indistinguishable from Hela by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Suspension
Morphology: Spherical
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 2-9×100, 000 cells/ml; 5% CO2; 37°C. To maintain cell growth in suspension cells should be grown in Spinner flasks (e.g. Techne Stirrers at speed of approx 40 rpm).
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1954;14:660; Ann NY Acad Sci 1958;76;497; Cancer Res 1955;15:598
Hyperlink to ECACC Cell Line Data Sheet: 85011412
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