Hep2 (HeLa derivative)

ECACC Cell Lines

CODE: 86030501

Human cervix carcinoma

[Create a profile and sign in to view pricing and add to cart]

Description
Ask a Question

Description

Description: Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Also Known As:

Species: Human

Tissue: Cervix

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. After thawing the first subculture may take 7-10 days.

Release Conditions: No

Depositor:

Originator: No

References: Cancer Res 1954;14:660; Cancer Res 1955;15:598; Soc Exp Biol Med 1956;193:107

Hyperlink to ECACC Cell Line Data Sheet: 86030501

Ask a Question

Have a question about this product? Our technical team is here to help.