HT29-MTX-E12
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Description
Description: HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and production of a mucous layer. HT29-MTX-E12 provides a model system to study the influence of the mucous layer on nanoparticle diffusion.
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Species: Human
Tissue: Colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO2; 37°C. Suggested seeding density 2-4×10,000 cells/cm².
Release Conditions: No
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Originator: No
References: Behrens I, Stenberg P, Artursson P, Kissel T. 2001 Transport of lipophilic drug molecules in a new mucus-secreting cell culture model based on HT29-MTX cells. Pharm Res. 18:1138-45. PMID: 11587485.
Hyperlink to ECACC Cell Line Data Sheet: 12040401
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