HT55
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Description
Description: Human colon epithelial cell line
Also Known As:
Species: Human
Tissue: Colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA and incubate until cell sheet peels off. Cells grow in islands, not monolayers; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 85061105
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