I-10
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Description
Description: Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 alpha-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).
Also Known As:
Species: Mouse
Tissue: testis
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Ham’s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Science 1966;154:1186; Endocrinology 1967;81:440
Hyperlink to ECACC Cell Line Data Sheet: 89031605
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