LLC-WRC 256
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Description
Description: Walker rat tumour cell line, which supports the growth of herpes simplex, pseudorabies and vaccinia viruses. The cells are highly tumourigenic in suckling rats.
Also Known As:
Species: Rat
Tissue: Unknown
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Medium 199 + 2mM Glutamine + 5% Horse Serum (HS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C
Release Conditions: No
Depositor:
Originator:
References: Science 1953;117-223; Anat Rec 1956;124:490
Hyperlink to ECACC Cell Line Data Sheet: 87061101
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