Description: Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) “, also known as CDw150, which is a receptor for measles virus. Clones of transfected cells were selected in the presence of geneticin (also known as G418). Vero/hSLAM cells can be used to isolate measles virus from human clinical samples and are a substitute for the B95a cell line for this purpose. Mumps susceptibility has also been demonstrated at the depositor's laboratory.

Also Known As:

Species: African green monkey

Tissue: Kidney

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: MEM with Earle's salts + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.4mg/ml Geneticin. It is essential that geneticin is added to the culture medium as it is required to maintain the expression of the SLAM gene.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union

Depositor:

Originator: No

References: Ono et al., 2001. Measles Viruses on Throat Swabs from Measles Patients Use Signaling Lymphocytic Activation Molecule (CDw150) but Not CD46 as a Cellular Receptor. Journal of Virology, 75;4399-4401

Hyperlink to ECACC Cell Line Data Sheet: 04091501