H129-19.6.8

CODE: 85103101
Mouse P3X63Ag8.653 x Rat LOU/WS1 spleen

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Description

Description: The antibody recognises a monomorphic determinant on the L3T4 molecule (the murine equivalent of CD4).

Also Known As:

Species: Mouse/Rat

Growth Medium: DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.

Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr M Pierres, Centre d'Immunologie, INSERM-CNRS, Marseilles

Originator: Yes

References: J Immunol 1984;132:6, 2775

Hyperlink to ECACC Cell Line Data Sheet: 85103101

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