ATC-10 (Aedes aegypti)
Mosquito larvae
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Description
Description: Derived from pooled newly hatched Aedes aegypti larvae. The cells are susceptible to a range of arboviruses.
Also Known As: Aedes aegypti
Species: Mosquito
Tissue: larvae
Growth Properties: Adherent aggregates
Morphology: Epithelial
Growth Medium: Mitsuhashi and Maramorosch Insect Medium (pH 7.0) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5 x10000 cells/cm² using 0.25% trypsin for 5 mins only 28°C. After quenching the trypsin with fresh mediumdislodge the cells by gentle scraping.
Release Conditions: No
Depositor:
Originator: No
References: Curr Sci 1967;36:506Curr Sci 1968;37:65
Hyperlink to ECACC Cell Line Data Sheet: 87091801
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