AFRC MAC 65

CODE: 86030313
Rat Y3Ag1.2.3 x Rat LOU/Iap spleen

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Description

Description: The antibody directed against peribacteroid membrane from Rhizobium-induced root nodules of Pisum sativum. The antigen is also found in plasma membranes and Golgi bodies, but not in endoplasmic reticulum ER. The antibody also reacts with peribacteroid membranes from nodules of Vicia and lupin.

Also Known As:

Species: Rat

Growth Medium: DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Release Conditions: No

Depositor: Dr G Galfre, AFRC Babraham Institute, Cambridge

Originator: Yes

References: EMBO J 1985;4(3):605

Hyperlink to ECACC Cell Line Data Sheet: 86030313

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