AKR-2B
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Description
Description: This cell line has the capacity to be activated to produce murine leukaemia virus (MLV). Mice of the high leukaemic inbred strain AKR regularly contain high levels of MLV throughout most of their post-natal life. However, studies of the AKR mouse embryo indicated that they contain very little, or no, infectious virus. Cell lines were found to produce virus only after several weeks in culture. Virus-negative cell lines can be induced to initiate synthesis of MLV (Virology 1971). Multiple transforming growth factors (TGFs) have been isolated from the serum-free conditioned media and acid-ethanol cell extracts of non-transformed AKR-2B cells.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: McCoy’s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:16 to 1:24 i.e. seeding at 4-10×1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow to reach confluence or grow over 20 passages.
Release Conditions: No
Depositor: Dr H Moses, Vanderbilt University School of Medicine, Tennessee
Originator: Yes
References: Virology 1971;46:866; Cancer Res 1983;43:1581
Hyperlink to ECACC Cell Line Data Sheet: 91061307
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