AsPC-1
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Description
Description: This cell line was derived from nude mouse xenografts initiated with cells from the ascites of a 62-year-old female Caucasian patient with cancer of the pancreas. The cells produce carcinoembryonic antigen (CEA)”, human pancreas associated antigen, human pancreas specific antigen and mucin.
Also Known As:
Species: Human
Tissue: Pancreas (ascites meta)
Growth Properties: Adherent
Morphology:
Growth Medium: RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Chen et al., 1982. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34.
Hyperlink to ECACC Cell Line Data Sheet: 96020930
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