B12
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Description
Description: Ethyl nitrosourea induced tumour cell line. Glial cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.
Also Known As:
Species: Rat
Tissue: nerve
Growth Properties: Adherent
Morphology: Glial
Growth Medium: DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Nature 1974;249:224
Hyperlink to ECACC Cell Line Data Sheet: 85042303
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