B92
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Description
Description: Ethyl nitrosaurea induced tumour cell line. Neuronal and glial cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Morphology significantly affected when grown in serum free media.
Also Known As:
Species: Rat
Tissue: nerve
Growth Properties: Adherent
Morphology: Glial/neuronal
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Nature 1974;249:224
Hyperlink to ECACC Cell Line Data Sheet: 85042304
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