Description: Derived from the aorta of a Friesian cow. Cells form “sprouts” after confluence. Infected with Bovine Viral Diarrhoea Virus (BVDV) which is a common contaminant of ruminants and is non-hazardous to humans. Secretes proteoglycans and collagen.

Also Known As:

Species: Bovine

Tissue: Aorta

Growth Properties: Adherent

Morphology: Endothelial

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. There is a tendancy for the cells to grow fibroblast-like until they are confluent. Once confluent the endothelial morphology prevails. Recent PHE Culture Collections investigations suggest that this cell line is starting to show signs of senescence. As this cell line is finite PHE Culture Collections do not recommend passaging these cells more than 10 times beyond the frozen stock.

Release Conditions: No

Depositor: Dr CI Levene. Department of Pathology, University of Cambridge

Originator: Yes

References: Macarak EJ, Kirby E, Kirk T, Kefalides NA (1978) Synthesis of cold-insoluble globulin by cultured calf endothelial cells. Proc Natl Acad Sci U S A.75(6):2621-5 PMID: 96438 .

Hyperlink to ECACC Cell Line Data Sheet: 88031149