BHK21C13-3P
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Description
Description: BHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (ECACC catalogue no. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.
Also Known As:
Species: Hamster
Tissue: Kidney
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Virology 1962;16:147
Hyperlink to ECACC Cell Line Data Sheet: 84100501
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