Bu (IMR-31)
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Description
Description: Derived from female Buffalo yearling. The cells have a finite life span of a minimum of 30 passages and are susceptible to a range of viruses including HSV, vaccinia, PRV, BVDV, para-influenza 3, bovine enterovirus (M62/4); also IBRV and VSV (Indiana). The cell line produces both detectable BVDV antigens and infectious BVDV.
Also Known As:
Species: Buffalo
Tissue: lung
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: McCoy’s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J. Virol. Methods 1994; 48: 211
Hyperlink to ECACC Cell Line Data Sheet: 89051704
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