BW1J
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Description
Description: A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice (see reference: Differentiation). Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli, fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.
Also Known As:
Species: Mouse
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Coon’s modified Ham’s F12 (or Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)
Subculturing Procedure: Split semi-confluent cultures (70%) 1:50 i.e. seeding at 2×1000 – 5×1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Media change three times a week.
Release Conditions: No
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris
Originator: Yes
References: Differentiation 1975; 4:85; Somatic Cell Genetics 1979; 5(6):719-738.
Hyperlink to ECACC Cell Line Data Sheet: 94101901
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