C1300 CLONE NA
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Description
Description: CLONE NA is a subclone of Neuro-2a, a clonal line of C1300 mouse neuroblastoma cells. The cells were selected for spontaneous mutations deficient in HGPRT in the presence of 8-azaguanine. This cell line has been mycoplasma eradicated at ECACC.
Also Known As:
Species: Mouse
Tissue: Neural
Growth Properties: Adherent
Morphology: Neuronal-like
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cultures may not form a complete monolayer before subculture is required.
Release Conditions: No
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia
Originator: Yes
References: Dev Biol 1974;39:226; J Clin Microbiol 1987;25:1456
Hyperlink to ECACC Cell Line Data Sheet: 93120817
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