Description: An immortalised mouse neural progenitor cell line capable of differentiation in vitro. The cell line was established by retorviral-mediated transduction of the avian myc oncogene into mitotic progenitor cells of neonatal mouse cerrebellum. Mouse strain CD1 x C57BL/6. This cell line is a valuable tool for in vitro and in vivo studies aimed at understanding the control of cell fate and differentiation of neural progenitors. The MMLV retrovirus vector used for the immortalisation process contained a neo resistance gene transcribed from an internal SV40 promoter. Therefore the cells are neo resistant. The morphology of the cells may change over time. Cells plated at low density may tend to become more process bearing whereas those plated more densely may tend to become flat and non-process bearing.

Also Known As:

Species: Mouse

Tissue: Cerebellum

Growth Properties: Adherent

Morphology: Neuronal

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Flasks should be pre-coated with poly-L-lysine at 10µg/ml in sterile distilled water.

Subculturing Procedure: Feed cells weekly with 50% conditioned medium and 50% fresh medium or split 1:10-1:20 in fresh medium using trypsin/EDTA. 5% CO2; 37°C. Split sub-confluent cultures 1:10 – 1:20 i.e. seeding at 2-4 x 10,000 cells/cm². Flasks should be pre-coated with poly-L-lysine at 10 micrograms/ml in sterile distilled water. Cells can be split as dilute as 1:50 , but the phenotype may change i.e. cells may appear flat with an epithelial-like morphology which usually do not stain for neurofilament or GFAP.

Release Conditions: No

Depositor: Constance L Cepko, Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston MA 02115

Originator: Yes

References: Ryder et al., (1990) Establishment and characterization of multipotent neural cell lines using retrovirus vector-mediated oncogene transfer. Journal of Neurobiology. 21,2, 356-375

Hyperlink to ECACC Cell Line Data Sheet: 07062902