C6-BU-1

CODE: 08062563
Rat glioma x rat glioma hybrid

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Description

Description: A 8-bromodeoxybromidine resistant mutant of the rat glioma cell line C6. Found to be tumourigenic in BALB/c nude mice and Wistar rats. Strain rattus norvegicus

Also Known As:

Species: Rat

Tissue: brain

Growth Properties: Adherent

Morphology: fibroblastic/fusiform

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C

Release Conditions: no

Depositor:

Originator: No

References: Amano et al.,” (1974) High activity of choline acetyltransferase iInduced in neuroblastoma x glia hybrid cells. Exp. Cell. Res. 85: 399-408.

Hyperlink to ECACC Cell Line Data Sheet: 08062563

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