CAR
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Description
Description: The cells support the growth of grunt fin agent with CPE at 20°C and 25°C. Also susceptible to infectious pancreatic necrosis virus of trout at 28°C.
Also Known As:
Species: Fish
Tissue: fin
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: BME (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin; 5% CO2; 25°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.
Release Conditions: No
Depositor:
Originator: No
References: Ann NY Acad Sci 1964;126:344
Hyperlink to ECACC Cell Line Data Sheet: 89072611
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