CHO-K1.Cl-0205
CHO-K1", Fc gamma RIIa , Fc gamma receptor 2A, 131H, allotype, human IgG ," IgG binding
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Description
Description: Clone of CHO-K1 cells expressing human FcgammaRIIa of the 131H allotype as a transmembrane protein with its native intracellular domain
Also Known As:
Species: Hamster
Tissue: Ovary
Growth Properties: Adherent
Morphology: Polygonal
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non- Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) with 600ug/ml hygromycin B
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10000 cells/cm2 using 0.05% trypsin/EDTA; culture conditions 5% CO2/95%air; 37°C
Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form
Depositor: Dr Kathryn L Armour
Originator: Yes
References: Armour KL, Smith CS, Turner CP, Kirton CM, Wilkes AM, Hadley AG, Ghevaert C, Williamson LM, Clark MR. Low-affinity Fc gamma R interactions can decide the fate of novel human IgG-sensitised red blood cells and platelets. Eur J Immunol. 2014Mar; 44 (3):905-14. doi: 10.1002/eji.201343825. Epub 2014 Feb 16. PubMed
Hyperlink to ECACC Cell Line Data Sheet: 15042905
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