CHO-K1.Cl-0270
CHO-K1", non-human primate Fc gamma RIIb , Fc gamma receptor 2b, Macaca mulatta, Rhesus macaque, Macaca fascicularis," Cynomolgus macaque
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Description
Description: Clone of CHO-K1 cells expressing * Macaca mulatta (Rhesus macaque) / * Macaca fascicularis (Cynomolgus macaque) FcgammaRIIb as a transmembrane protein. * The sequence of the extracellular domains of FcgammaRIIb from Rhesus macaque (NP_001258577) and a predicted sequence for the one polymorphic form of FcgammaRIIb from Cynomolgus macaque (NW_005092960) are identical at the amino acid level. There is one amino acid change (Leu104Met) relative to the Cynomolgus macaque sequence AAL92097
Also Known As:
Species: Hamster
Tissue: Ovary
Growth Properties: Adherent
Morphology: Polygonal
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non- Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) with 600ug/ml hygromycin B
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10000 cells/cm2 using 0.05% trypsin/EDTA; culture conditions 5% CO2/95%air; 37°C
Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form
Depositor: Dr Kathryn L Armour
Originator: Yes
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 15042912
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