Clone 1-5c-4 (HeLa deriative)
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Description
Description: These cells were originally Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival ‘D’ cells. Susceptible to swine influenza viruses, supports production of plaque formation by a variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Epithelial with occasional giant cells. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Medium 199 (HBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using EDTA or 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Exp Med 1961;95:113; In Vitro 1978;14:469; Proc Soc Exp Biol Med 1954;87:440; Nature 1976;259:211
Hyperlink to ECACC Cell Line Data Sheet: 88021103
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