Description: Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific for non-structural protein NS3 (helicase domain). This antibody binds to a major conformational epitiope located at the carboxyl-terminal end of the NS3 region that is recognised by approximately ~90% from HCV-infected patients. CM3.B6 can be used in immunochemistry, HCV protein purification and HCV typing. CM3.B6 and B12.F8 (ECACC Catalogue number 11060102) were derived from the same patient as confirmed by STR profiling.

Also Known As:

Species: Human

Tissue: Blood

Growth Properties: Suspension

Morphology: Small rounded cells

Growth Medium: RPMI-1640 + 2mM L-Glutamine + 1% Non-Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS) + 20U/ml Interlukin-6 (IL-6).

Subculturing Procedure: Maintain cultures between 3-9 x 105/ml. 5% CO2; 37°C. Media change every two days. Saturation density 1.1 x 106/ml

Release Conditions: Yes – CM3.B6 CANNOT be released without permission from the depositor. Please complete attached cell line release authorisation form.

Depositor: Mario U Mondelli, MD PhD FRCP, Professor of Infectious Diseases, University of Pavia, Director, Research Laboratories and Hepatology Centre, Department of Infectious Diseases, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Originator: Yes

References: Mondelli MU, Cerino A, Boender P, Oudshoorn P, Middeldorp J, Fipaldini C, La Monica N, Habets W 1994 Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody. J Virol. 68(8):4829-36 PMID: 7518528.

Hyperlink to ECACC Cell Line Data Sheet: 11060101