COLO 853
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Description
Description: Derived from the right axilla lymph node of a 43 year old male with melanoma. COLO 839 (ECACC Catalogue number 93051119)”, COLO 858 (ECACC Catalogue number 93052613) and COLO 857 (ECACC Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma. This has been confirmed by STR profiling; COLO 853 also has an identical STR profile to the above cell lines though this cell line is not mentioned in the literature (Morse HG, Moore GE PMID: 8402545, Easty DJ et al., PMID: 7780963 and Maung K et al PMID: 10597292).
Also Known As:
Species: Human
Tissue: lymph node
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507
Originator: Yes
References: Unpublished
Hyperlink to ECACC Cell Line Data Sheet: 93052606
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