Description: COS-1 cell line (ECACC catalogue no. 88031701) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent i.e. adherent. COS-1 was derived from CV-1 simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.

Also Known As:

Species: Monkey

Tissue: Kidney

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: InVitrus medium (Cell Culture Technologies http://www.cellculture.com/) + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Subculturing Procedure: In order to prevent syncitia formation, do not allow cells to become confluent. Split semi-confluent cultures 1:3 i.e. seeding at 4×10,000 cells/cm² using an animal component free dissociation reagent; incubate at 37°C. Please note: Once cells have detached from the flask it is necessary to neutralise the dissociation reagent using a compatible inhibitor . Centrifuge the cells; 100-150 x g for 3-5 minutes; count and re-suspend at the recommended seeding density in fresh medium

Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union

Depositor: Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies LLC, Via al Chioso 12, 6929 Gravesano, Switzerland, and Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland. Supported by the Swiss Foundation FFVFF

Originator: Yes

References: None Specified By Depositor

Hyperlink to ECACC Cell Line Data Sheet: 04092902