D98/AH2 Clone B (HeLa derivative)
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Description
Description: This cell line has been shown to be resistant to 8-azaguanine. The cell line was recloned in the depositor’s laboratory and is useful in transformation studies. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1962;48:2026; Nat Cancer Inst 1962;1:75; Science 1969;163:697
Hyperlink to ECACC Cell Line Data Sheet: 85112701
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