DBTRG.05MG

CODE: 93061119
Human glioblastoma

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Description

Description: Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.

Also Known As:

Species: Human

Tissue: Brain

Growth Properties: Adherent

Morphology: Glial

Growth Medium: RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr C Kruse, University of Colorado Health Sciences Center, Denver, Co 80262

Originator: Yes

References: In Vitro 1992;28A:609

Hyperlink to ECACC Cell Line Data Sheet: 93061119

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