DBTRG.05MG
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Description
Description: Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.
Also Known As:
Species: Human
Tissue: Brain
Growth Properties: Adherent
Morphology: Glial
Growth Medium: RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr C Kruse, University of Colorado Health Sciences Center, Denver, Co 80262
Originator: Yes
References: In Vitro 1992;28A:609
Hyperlink to ECACC Cell Line Data Sheet: 93061119
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