Detroit 510
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Description
Description: Derived from skin tissue of a 9 month old Caucasian female with Galactosemia. Galactose-1-phosphate transferase negative.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10%Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Finite lifespan, up to 30 population doublings are expected.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1965;53:350; Science 1965;148:42; Science 1972;175:1368
Hyperlink to ECACC Cell Line Data Sheet: 90102531
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