EIII
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Description
Description: This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.
Also Known As:
Species: Human/mouse hybrid
Tissue: hybrid
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Release Conditions: No
Depositor: Dr P Scambler, Institute of Child Health, London
Originator: Yes
References: Nucleic Acids Res 1986;14:7159
Hyperlink to ECACC Cell Line Data Sheet: 86072804
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