EJG
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Description
Description: The bovine cell line was established from collagenase-dissociated, adrenal medulla tissue. The cells display a capillary endothelium morphology with relatively large cell bodies and produce factor VIII:C. Tests for BVDV have shown that the cell line is producing both detectable BVDV antigens and infectious BVDV.
Also Known As:
Species: Bovine
Tissue: adrenal
Growth Properties: Adherent
Morphology: Endothelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Passage cells every 5 to 7 days.
Release Conditions: No
Depositor:
Originator: No
References: J. Virol. Methods 1994;48:211
Hyperlink to ECACC Cell Line Data Sheet: 93051803
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