Description: Peripheral blood lymphocytes from a healthy male Tunisian, immortalised by EBV transformation. The cell line contains the following deletion, at the homozygous state, in the human immunoglobulin heavy locus (IGH on chromosome 14 at 14q32.33), of 6 IGHC genes: on both chromosomes 14, deletion I (del G1-EP1-A1-GP-G2-G4). The Gm-Am genotype is Gm5*; – ; – A2m2/Gm5*; – ; – A2m2 (Gm b*; – ; – A2m2/Gm b*; – ; – A2m2). Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4.

AAn Epstein-Barr-transformed lymphoblastoid cell line is available, from the mother heterozygous for deletions I and II, as TOU (TOU I-2) (93093001) and, from one brother heterozygous for deletions I and II, as MOH (TOU II-3) (93093002).An Epstein-Barr-transformed lymphoblastoid cell line with an identical deletion I, at the homozygous state, is available, from one member of another Tunisian family, as TAK3 (93093004).

Also Known As:

Species: Human

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblast

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures at 5-9×100,000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Prof M-P Lefranc and Prof G Lefranc, Laboratoire d'ImmunoGenetique Moleculaire (LIGM), Universite de Montpellier

Originator: Yes

References: Lefranc M-P, Lefranc G, Rabbitts TH. 1982 Inherited deletion of immunoglobulin heavy chain constant region genes in normal human individuals. Nature 300(5894):760-2. PMID: 6817143

Hyperlink to ECACC Cell Line Data Sheet: 93093003