F9
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Description
Description: Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology:
Growth Medium: DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2, 37°C. Upon resuscitation, cells shed large quantities of debris, leave for 2 days before subculture. Please note: Culture vessels must be coated with 0.1% gelatin prior to use, pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.
Release Conditions: No
Depositor:
Originator: No
References: Proc Nat Acad Sci, USA 1973;70:3899; Cell 1978;15:393; Cell 1980;21:347
Hyperlink to ECACC Cell Line Data Sheet: 85061803
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