G-361
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Description
Description: Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: McCoy’s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells are slow in attaching.
Release Conditions: No
Depositor:
Originator: No
References: Paediatric Res 1978;12:485
Hyperlink to ECACC Cell Line Data Sheet: 88030401
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